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mys 10 il 15  (InvivoGen)


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    InvivoGen mys 10 il 15
    Mys 10 Il 15, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 686 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mys+10+il+15/MycoStrip+2%2E0/pm41734064-265-9-8
    Average 99 stars, based on 686 article reviews
    mys 10 il 15 - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Isolation:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17 ..

    Staining:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17 ..

    Virus:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17 ..

    RNA sequencing:

    Article Title: Glutathione is critical for NK cell-mediated immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 7-ADD (1:100) Thermo Fisher Scientific # A1310 CD107a-PE Clone 1D4B (1:200) Thermo Fisher Scientific #15248639 CD11b-Pacific Blue Clone M1/70 (1:200) Biolegend #101224 CD11b-APC-eFluor780 Clone M1/70 (1:200) Thermo Fisher Scientific #15391420 CD19-PE Clone 1D3 (1:200) Biolegend #152407 CD19-APC-eFluor 780 Clone 1D3 (1:200) Thermo Fisher Scientific #15331430 CD27-FITC Clone LG.3A10 (1:200) Biolegend #124207 CD3ε-APC/Cy7 Clone 145-2C11 (1:200) Biolegend #100330 CD3ε-APC-eFluor 780 Clone 17A2 (1:200) Thermo Fisher Scientific #15311420 CD336 (TIM-3)-BV785 Clone RMT3-23 (1:200) Biolegend #119725 CD44-PE/Cy7 Clone IM7 (1:200) Thermo Fisher Scientific #15370920 CD49b-BV785 Clone HMα2 (1:200) Biolegend #103518 CD4-BV785 Clone GK1.5 1:200) Biolegend #100453 CD5-APC-eFluor 780 Clone 53–7.3 (1:200) Thermo Fisher Scientific #15539506 CD62L-Pacific Blue Clone MEL-14 (1:200) Biolegend #104424 CD8α-BUV-395 Clone 53–6.7 (1:200) BD Biosciences #563786 Granzyme B-APC Clone QA16A02 (1:200) Biolegend #372203 IFN-γ-APC Clone XMG1.2 (1:200) Biolegend #505810 Ki67-PE Clone 11F6 (1:200) Biolegend #151209 Ly6G/Ly6C-APC-eFLuor780 Clone RB6-8C5 (1:200) Thermo Fisher Scientific #15391660 NK1.1-APC Clone PK136 (1:200) Biolegend #108710 NK1.1-PE Clone PK136 (1:200) Biolegend #108708 NKG2D-FITC Clone C7 (1:200) Biolegend #115711 PD-1-BV605 Clone 29F.1A12 (1.200) Biolegend #135220 pmTOR S2448-eFluor450 Clone MRRBY (1:200) Thermo Fisher Scientific #15549836 pS6 S235/S236-APC Clone cupk43k (1:200) Thermo Fisher Scientific #15528216 Puromycin-AF488 Clone 12D10 (1:200) Merck/Sigma Aldrich #MABE343-AF488 p-STAT5 (Y694)-FITC Clone [Stat5Y694-G11] (1:200) Abcam #ab278791 TER119-APC-eFluor780 Clone TER-119 (1:200) Thermo Fisher Scientific #15371660 TIGIT (Vstm3)-PE Clone 1G9 (1:200) Biolegend #142104 Chemicals, peptides, and recombinant proteins N-Acetyl-L-cysteine (NAC) Sigma-Aldrich #A7250-100G L-Buthionine-sulfoximine Sigma-Aldrich #B2515 PBS (1X) without Ca++, Mg++, 500mL Westburg #BE17-516F FBS Superior Lot: 0193F Biochrom GmbH #S0615 Penicillin-Streptomycin (10,000 U/mL) Gibco #11548876 L-Glutamine Westburg #BE17-605E 2-Mercaptoethanol Gibco #11508916 Glutamax Thermo Fisher Scientific #35050061 Gibco TM Sodium Pyruvate Thermo Fisher Scientific #12539059 EDTA Sigma-Aldrich #EDS-100G Bovine Serum Albumin (BSA) Sigma-Aldrich #A9647-500G RPMI 1640 (without L-Glutamine) Westburg #BE12-167F IMDM with HEPES and L-Glutamine Westburg #BE12-722F (Continued on next page) 16 Cell Reports 45, 116986, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER MycoStrip_Mycoplasma detection kit Invivogen #rep-mys-10 IL-15, mouse Peprotech #210-15 Formaldehyde Sigma-Aldrich #252549-1L Saponin Sigma-Aldrich #S4521-25G Monobromobimane (mBBr) Thermo Fisher Scientific #M1378 H2DCFDA (1:2000) Thermo Fisher Scientific #D399 Hoechst 33342, Tocris Bioscience TM Thermo Fisher Scientific #30002028 H-2D(b) LCMV GP var C41M 33–41 KAVYNFATM NIH Tetramer Facility N/A H-2D(b) LCMV NP 396–404 FQPQNGQFI NIH Tetramer Facility N/A I-A(b) LCMV GP 66–77 DIYKGVYQFKSV NIH Tetramer Facility N/A GP 33-41 KAVYNFAT GenScript (Hong Kong) N/A NP 396-404 FQPQNGQFI GenScript (Hong Kong) N/A GP 66-77 DIYKGVYQFKSV GenScript (Hong Kong) N/A MitoSOX TM Red Mitochondrial Superoxide Indicator (1:5000) Thermo Fisher Scientific #M36008 MitoTracker TM Deep Red FM (1:10.000) Thermo Fisher Scientific #M22426 MitoTracker Green FM (1:100.000) Thermo Fisher Scientific #M7514 DAPI (1:2000) Thermo Fisher Scientific #D1306 Zombie NIR TM Fixable Viability Kit (1:1000) Biolegend #423106 Zombie Green TM Fixable Viability Kit (1:1000) Biolegend #423112 Oligomycin A Sigma-Aldrich #75351-5MG FCCP Sigma-Aldrich #C2920-10MG Antimycin A Sigma-Aldrich #A8674-25MG Rotenone Sigma-Aldrich #R8875-1G Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich #P8139-1MG Streptavidin APC Biolegend #405207 Calcium Ionophore A23187 Sigma-Aldrich #C7522-1MG GolgiPlug TM (Protein Transport Inhibitor) BD Biosciences #555029 RNase Thermo Fisher Scientific #R1253 Critical commercial assays NK Cell Isolation Kit, mouse Miltenyi Biotec #130-115-818 Tumor Dissociation Kit, mouse Miltenyi Biotec #130-096-730 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific #15151976 BD Cytofix/Cytoperm Fixation/Permeabilization kit BD Biosciences #554714 IFN gamma Mouse Uncoated ELISA Invitrogen TM #15501107 GSH/GSSG-Glo TM Assay Promega #V6612 CellTiter-Glo® Promega #G7571 RNeasy Micro Kit Qiagen #74004 SPOTCHEM II LIVER-1 ARKRAY #77182 PTK analysis kit Pamgene #86402 STK analysis kit Pamgene #87102 Experimental models: Organisms/strains Gclc fl/fl : B6 Mak et al. 9 N/A NCR1-Cre: B6 Narni-Mancinelli at al. 34 N/A Lymphocytic choriomeningitis virus WE – N/A B16-F10 – N/A MC-38 – N/A Deposited data RNA-Seq This manuscript GEO: GSE290323 (Continued on next page) Cell Reports 45, 116986, March 24, 2026 17 ..



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